Pulsed-field gel electrophoresis of very large DNA molecules.
نویسندگان
چکیده
FACTORS THAT AFFECT PFG SEPARATIONS........................................................................ 294 Molecular Properties ............................................... .................................... ............. 294 Pulse Times............................................................................................................... 295 Electrical Field Shape ............................................................................................... 296 Electrical Field Strength ........................................................................................... 297 Gel Concentration.......... ............................................ ............................................... 298 DNA Concentration ..................................................................................... ............. 299 Temperature ................................................ . . . ............................ ................. ............. 299 THEORETICAL ANALYSIS OF PFG ................................................................................ ...... 300 DNA Shape ............................................. ................................................................. 300 DNA Reorientation................................................................................................... 301
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This section describes the application of agarose gel electrophoresis to both analytical and preparative separation of DNA fragments. Standard agarose gels separate DNA fragments from ∼0.5 to 25 kb, whereas pulsed-field agarose gels resolve molecules from ∼10 to >2000 kb. Descriptions of standard and pulsed-field agarose gel electrophoresis as well as parameters affecting resolution of large DN...
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ورودعنوان ژورنال:
- Annual review of biophysics and biophysical chemistry
دوره 17 شماره
صفحات -
تاریخ انتشار 1988